SCREEN IFA TEST PROCALCITONINA

Fluorescence Immunoassay Procalcitonin

Product code: SC-1392-20

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Fluorescence Immunoassay Procalcitonin – Product code: SC-1392-20

Test for the diagnosis of inflammatory state by measuring Procalcitonin (PCT) on whole blood, serum or plasma with the use of the Immunofluorescence Analyzer. The PCT Cassette Test (Whole Blood/Serum/Plasma) is an immunofluorescence-based test for the quantitative detection of human procalcitonin on whole blood, serum or plasma as an aid in the diagnosis of inflammatory state. Procalcitonin (PCT) is a small protein comprising 116 amino acid residues with a molecular weight of approximately 13 kDa, first described by Moullec et al. in 1984. PCT is normally produced in the C cells of the thyroid glands. In 1993 a high level of PCT was observed in patients with systemic bacterial infection and today PCT is considered the main marker of disorders accompanied by systemic inflammation and sepsis. The diagnostic value of PCT is important given the close correlation between its concentration and the severity of the inflammation. It has been shown that “inflammatory” PCT is not produced by C cells. The source of PCT during inflammation appears to be cells of neuroendocrine origin. The PCT Cassette Test (Whole Blood/Serum/Plasma) is based on immunofluorescence. The sample moves across the strip from the sample pad to the absorbent pad. If the sample contains Procalcitonin, it attaches to antibody-conjugated fluorescent beads. Then the compound is captured by the capture antibodies placed on the nitrocellulose membrane (Test line). The concentration of PCT in the sample is directly proportional to the intensity of the fluorescent signal captured on the T-line. Depending on the intensity of the fluorescence on the test and the standard curve, the concentration of PCT in the sample is calculated by the SCREEN® Analyzer to show the PCT concentration in the sample.

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