Campylobacter Rapid Test – Product code: SC-0463-10
Rapid test for the qualitative detection of Campylobacter in human stool samples. For professional in vitro diagnostic use only. The Campylobacter Rapid Cassette Test (Feces) is based on the use of a membrane technology with colloidal gold. A nitrocellulose membrane is sensitized with a direct anti-Campylobacter antibody. The specificity of the test is guaranteed by a specific antibody for a Campylobacter antigen conjugated with colloidal gold. Campylobacter enteritis is a zoonotic disease, transmitted through food and water, and is one of the most common causes of infectious diarrhea in the United States. While identification of the causative agent does not typically affect treatment outcomes, as most of these infections are self-limited, laboratory diagnosis is essential for epidemiological studies and for monitoring the outbreak through identification. and strain typing. Conventional laboratory diagnosis of campylobacteriosis is based on the recovery of the organism from stool samples by microaerophilic culture. Current recommendations regarding Campylobacter recovery require cultures to be stored for a minimum of 72 hours before confirming a negative result ; however, recent laboratory monitoring by the Centers for Disease Control and Prevention found that, of the laboratories surveyed, 66% reported negative results at 48 hours, while only 33% reported negative results after 72 hours . Unlike traditional culture methods, the fastest methods for detecting Campylobacter antigens in stool, including enzyme immunoassay (EIA) and lateral flow systems, take only 1 to 2 hours to obtain results. The Campylobacter Rapid Cassette Test (Feces) is based on the use of membrane technology with colloidal gold. A nitrocellulose membrane is sensitized with a direct anti-Campylobacter antibody. The specificity of the test is guaranteed by a specific antibody for a Campylobacter antigen conjugated with colloidal gold. This conjugate is dried on a membrane. The fecal sample must be diluted in the extraction buffer provided with the test kit. When the extracted sample contacts the test strip, the conjugate migrates with the sample by passive diffusion and the conjugate and sample material contact the anti-Campylobacter antibody in the test line (T). If the sample contains Campylobacter antigen, the conjugate-antigen complex will remain bound to the anti-Campylobacter reagent and a colored line will develop. The solution continues to migrate to encounter a second reagent that binds the migration control conjugate, thus producing a colored control line that confirms that the assay is functioning correctly. The result is visible within 10 minutes.