SCREEN IFA TEST TSH

Fluorescence Immunoassay Thyroid Stimulating Hormone

Product code: SC-1378-20

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Fluorescence Immunoassay Thyroid Stimulating Hormone – Product code: SC-1378-20

Rapid test for the quantitative detection of TSH in serum or plasma through the use of the immunofluorescence analyzer. The TSH Cassette Test (Immunofluorescence Test) is designed for the in vitro quantitative determination of Thyrostimulating Hormone (TSH) in serum or plasma. The measurement of TSH is useful to assist in the screening of primary hypothyroidism in the adult population by health professionals. It can also be used in the screening of neonatal hypothyroidism. Thyroid stimulating hormone (also known as thyrotropin, thyrotropic hormone, TSH or hTSH which stands for human TSH) is a pituitary hormone that stimulates the thyroid gland to produce tyrosine (T4) and subsequently triiodothyronine (T3) which stimulates the metabolism of almost everyone body tissues 1. It is a glycoprotein hormone synthesized and secreted by thyrotrophic cells in the anterior pituitary gland that regulates the endocrine functions of the thyroid. Thyroid stimulating hormone (also known as thyrotropin, thyrotropic hormone, TSH or hTSH which stands for human TSH) is a pituitary hormone that stimulates the thyroid gland to produce tyrosine (T4) and subsequently triiodothyronine (T3) which stimulates the metabolism of almost everyone body tissues 1. It is a glycoprotein hormone synthesized and secreted by thyrotrophic cells in the anterior pituitary gland that regulates the endocrine functions of the thyroid. TSH (with a half-life of about one hour) stimulates the thyroid gland to secrete the hormone tyrosine (T4) which has only a mild effect on metabolism. T4 is then converted into triiodothyronine (T3) which is the active hormone that stimulates metabolism. About 80% of this conversion occurs in the liver and other organs, and 20% in the thyroid itself 1. Laboratory tests on the levels of thyroid stimulating hormone * in the blood are considered the best initial test for hypothyroidism4. It is important to remember the statement of the Subclinical Thyroid Disease consensus Panel (Conference on Subclinical Thyroid Pathology): “There is no single level of TSH serum for which clinical intervention is always indicated or contraindicated. The higher the TSH, the more urgent the therapeutic logic will be. It is important to consider the individual clinical context (eg pregnancy, lipid profile, ATPO antibodies) “5. The TSH SCREEN® Cassette Test is based on immunofluorescence technology. The TSH SCREEN® test cassette (Serum/Plasma) identifies the Thyrostimulating hormone through an immunochromatographic quantitative detection technology. The sample moves across the strip from the sample buffer to the absorbent pad by chromatographic action. If the sample contains TSH, it attaches to the fluorescent beads conjugated to TSH antibodies. Then the compound is captured by the capture antibodies placed on the nitrocellulose membrane (Test line). The concentration of TSH in the sample is directly proportional to the intensity of the fluorescent signal captured on the T-line. Depending on the intensity of the fluorescence on the test and the standard curve, the concentration of TSH in the sample is calculated by the SCREEN® Analyzer to show the TSH concentration in the sample.

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