Fluorescence Immunoassay Troponin I – Product code: SC-1590-20
Rapid test for the diagnosis of myocardial infarction (MI) to detect cardiac Troponin I (cTnl) in whole blood, serum or plasma with the use of the Screen® Immunofluorescence Analyzer. The Cardiac Troponin I Cassette Test (Whole Blood / Serum / Plasma) identifies cardiac Troponin I (cTnl) through immunofluorescence. The sample moves across the strip from the sample pad to the absorbent pad. If the sample contains cTnl, it attaches to anti-cTnl antibodies conjugated with fluorescent beads. Then the compound is captured by the capture antibodies that cover the nitrocellulose membrane (Test line). The concentration of cTnl in the sample is directly related to the intensity of the fluorescent signal captured on the T-line. Depending on the fluorescence intensity of the test and the standard curve of the product, the concentration of cTnl in the sample can be calculated by the Screen® Reader for indicate the concentration of cTnl in the sample. Cardiac Troponin I (cTnl) is a protein found in heart muscle with a molecular weight of 22.5 kDa [1]. Troponin I is part of a three-subunit complex comprising Troponin T and Troponin C. Together with tropomyosin, this structural complex forms the main component regulating the calcium-dependent activity of actomyosin ATPase in striated and skeletal muscle tissue. cardiac. Following heart injury, Troponin I is released into the blood 4-6 hours after the onset of pain. The release pattern of cTnl is similar to that of CK-MB, but, while CK-MB levels return to normal after 72 hours, Troponin I remains elevated for 6-10 days, providing a larger window for detection of heart injuries. The high specificity of cTnl measurements for the identification of myocardial damage has been demonstrated in conditions such as the perioperative period, after a marathon and following severe thoracic trauma [3]. The release of cTnI has also been documented in cardiac conditions other than acute myocardial infarction (AMI) such as unstable angina, congestive heart failure and ischemic damage due to coronary artery bypass surgery. Due to its high specificity and sensitivity in myocardial tissue, Troponin I has recently become the preferred biomarker for myocardial infarction. The Cardiac Troponin I Cassette Test (Whole Blood / Serum / Plasma) is a simple test that uses a combination of anti-cTnl antibody coated particles and capture reagent to detect cTnl in whole blood, serum or plasma.
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